导师风采
李金明
个人信息
Personal Information
- 性别：男
- 导师类型：博士生导师
- 职称：研究员
- 该职称任职时间：20010701
联系方式
Contact Information
- 所属院系：国家卫生健康委临床检验中心
- 所属专业：临床检验诊断学
- 邮箱 : jmli@nccl.org.cn
- 工作电话 : 01058115061
个人简介
Personal Profile
1980.09-1983.06 湖南邵阳卫校学习1983.07-1986.07 航天部第722医院工作1986.08-1989.07 吉林医学院临床检验诊断学硕士研究生学习1989.08-1990.07 吉林医学院临床免疫研究室工作1990.08-1993.06 中国协和医科大学研究生院临床检验诊断学博士研究生学习1993.07-北京医院（国家卫生健康委临床检验中心）工作
团队简介
Team Profile
待补充更新
- 研究方向Research Directions
临床分子诊断方法及标准化
- 在校研究生Current Graduate Students
硕士研究生 0 名，博士研究生 4 名
- 科研项目Research Projects
| # | 项目名称 | 起止日期 | 金额 | 项目类型 | 本人角色 |
|---|---|---|---|---|---|
| 1 | 人体表型解析技术临床应用的标准化研究 | 2023-12 —— 2028-11 | 439.38万元 | 主持在研的国家或省部级科研项目 | 主持人 |
- 发表论文Papers
| # | 论文题目 | 期刊名称 | 发表年份 | 论文署名 |
|---|---|---|---|---|
| 1 | Diagnostic performance of mpox virus (MPXV) real-time PCR assays: multicenter assessment and extended sensitivity analysis | Eur J Clin Microbiol Infect Dis. | 2024-06 | 共同通讯 |
| 2 | Artificial intelligence in the clinical laboratory | Clin Chim Acta | 2024-05 | 共同通讯 |
| 3 | Harmonizing tumor mutational burden analysis: Insights from a multicenter study using in silico reference data sets in clinical whole-exome sequencing (WES) | Am J Clin Pathol | 2024-05 | 共同通讯 |
| 4 | External quality assessment for molecular detection of Ureaplasma urealyticum in China | Clin Chim Acta | 2024-03 | 共同通讯 |
| 5 | Enhancing the quality of panel-based tumor mutation burden assessment: a comprehensive study of real-world and in-silico outcomes | NPJ Precis Oncol | 2024-01 | 共同通讯 |
| 6 | CRISPR-Cas system: A promising tool for rapid detection of SARS-CoV-2 variants | J Med Virol | 2024-01 | 共同通讯 |
| 7 | RLP system: A single-tube two-step approach with dual amplification cascades for rapid identification of EGFR T790M | Anal Chim Acta | 2024-01 | 共同通讯 |
| 8 | External quality assessment for detection of colorectal cancer by Septin9 DNA methylation in clinical laboratories | Clin Chim Acta | 2024-01 | 共同通讯 |
| 9 | Unlocking the secrets: the power of methylation-based cfDNA detection of tissue damage in organ systems | Clin Epigenetics | 2023-10 | 通讯作者 |
| 10 | Multi-omics data integration using ratio-based quantitative profiling with Quartet reference materials | Nat Biotechnol | 2023-09 | 共同通讯 |
| 11 | Comparison of analytical sensitivity of DNA-based and RNA-based nucleic acid amplification tests for reproductive tract infection pathogens: implications for clinical applications | Microbiol Spectr | 2023-08 | 共同通讯 |
| 12 | Evaluation of noninvasive prenatal screening for copy number variations among screening laboratories | Clin Biochem | 2023-08 | 共同通讯 |
| 13 | Validation and benchmarking of targeted panel sequencing for cancer genomic profiling | Am J Clin Pathol | 2023-11 | 共同通讯 |
| 14 | Performance evaluation of SARS-CoV-2 antigen detection in the post-pandemic era: multi-laboratory assessment | Clin Chem Lab Med | 2023-06 | 共同通讯 |
| 15 | Assessing the Quality of Metagenomic Next-Generation Sequencing for Pathogen Detection in Lower Respiratory Infections | Clin Chem | 2023-09 | 共同通讯 |
| 16 | Off the fog to find the optimal choice: Research advances in biomarkers for early diagnosis and recurrence monitoring of bladder cancer | Biochim Biophys Acta Rev Cancer | 2023-07 | 共同通讯 |
| 17 | Emergence of CRISPR/Cas9-mediated bioimaging: A new dawn of in-situ detection | Biosens Bioelectron | 2023-07 | 共同通讯 |
| 18 | Analysis of Clinical Laboratory Detecting Challenging Variants from Exome Sequencing Using Simulated Patient-Parent Trio Sample: Pilot Study for Neurodevelopmental Disorder de Novo Variants | J Mol Diagn | 2023-06 | 通讯作者 |
| 19 | Circulating HPV DNA in HPV-associated cancers | Clin Chim Acta | 2023-03 | 通讯作者 |
| 20 | CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives | J Adv Res | 2023-08 | 共同通讯 |
| 21 | Focus on the frontier issue: progress in noninvasive prenatal screening for fetal trisomy from clinical perspectives | Crit Rev Clin Lab Sci | 2023-06 | 共同通讯 |
| 22 | Effectiveness of SARS-CoV-2-inactivated vaccine and the correlation to neutralizing antibodies: A test-negative case-control study | J Med Virol | 2023-01 | 共同通讯 |
| 23 | Consistency and reproducibility of large panel next-generation sequencing: Multi-laboratory assessment of somatic mutation detection on reference materials with mismatch repair and proofreading deficiency | J Adv Res | 2023-02 | 共同通讯 |
| 24 | Validation of a Metagenomic Next-Generation Sequencing Assay for Lower Respiratory Pathogen Detection | Microbiol Spectr | 2023-02 | 共同通讯 |
| 25 | Evaluating the value of anti-SARS-CoV-2 antibody detection and neutralizing responses with euvirus: A population of 10776 close contacts in the epidemic of Fujian | Clin Chim Acta | 2023-01 | 共同通讯 |
| 26 | Evaluation of Four Strategies for SARS-CoV-2 Detection: Characteristics and Prospects | Microbiol Spectr | 2022-12 | 共同通讯 |
| 27 | Carrier screening: An update | Clin Chim Acta | 2022-08 | 通讯作者 |
| 28 | A multi-laboratory assessment of clinical exome sequencing for detection of hereditary disease variants: 4441 ClinVar variants for clinical genomic test development and validation | Clin Chim Acta | 2022-08 | 通讯作者 |
| 29 | CRISPR/Cas9-mediated epigenetic editing tool: An optimized strategy for targeting de novo DNA methylation with stable status via homology directed repair pathway | Biochimie | 2022-11 | 共同通讯 |
| 30 | Reprogramming of Human B Cells from Secreting IgG to IgM by Genome Editing | CRISPR J | 2022-10 | 通讯作者 |
| 31 | Nomogram Model for Prediction of SARS-CoV-2 Breakthrough Infection in Fujian: A Case-Control Real-World Study | Front Cell Infect Microbiol | 2022-06 | 共同通讯 |
| 32 | A national proficiency scheme for human leucocyte antigen typing by next-generation sequencing | Clin Chim Acta | 2022-06 | 通讯作者 |
| 33 | Asporin Interacts With HER2 to Promote Thyroid Cancer Metastasis via the MAPK/EMT Signaling Pathway | Front Oncol | 2022-05 | 共同通讯 |
| 34 | Continual Improvement of the Reliability of Next-Generation Sequencing-Based ctDNA Analysis: A Long-Term Comparison of ctDNA Detection in China | Clin Chem | 2022-07 | 共同通讯 |
| 35 | Development of a Novel Reference Material for Tumor Mutational Burden Measurement Based on CRISPR/Cas9 Technology | Front Oncol | 2022-04 | 通讯作者 |
| 36 | Liquid biopsy for early diagnosis of non-small cell lung carcinoma: recent research and detection technologies | Biochim Biophys Acta Rev Cancer | 2022-04 | 共同通讯 |
| 37 | Design, optimization, and application of multiplex rRT-PCR in the detection of respiratory viruses | Crit Rev Clin Lab Sci | 2022-05 | 共同通讯 |
| 38 | Metagenomics next-generation sequencing tests take the stage in the diagnosis of lower respiratory tract infection | J Adv Res | 2022-06 | 共同通讯 |
| 39 | Multilaboratory assessment of metagenomic next-generation sequencing for unbiased microbe detection | J Adv Res | 2022-06 | 共同通讯 |
| 40 | Impact of SARS-CoV-2 Variants on the Analytical Sensitivity of rRT-PCR Assays | J Clin Microbiol | 2022-03 | 共同通讯 |
| 41 | Adaptation of ACMG-ClinGen Technical Standards for Copy Number Variant Interpretation Concordance | Front Genet | 2022-03 | 通讯作者 |
| 42 | Non-invasive prenatal screening for foetal trisomy: An assessment of reliability and reporting | Clin Biochem | 2022-02 | 共同通讯 |
| 43 | Preparation of multiplexed control materials for cancer mutation analysis by genome editing in GM12878 cells | J Clin Lab Anal | 2022-01 | 通讯作者 |
| 44 | CircMTO1 suppresses hepatocellular carcinoma progression via the miR-541-5p/ZIC1 axis by regulating Wnt/β-catenin signaling pathway and epithelial-to-mesenchymal transition | Cell Death Dis | 2021-12 | 共同通讯 |
| 45 | External quality assessment of molecular testing of 9 viral encephalitis-related viruses in China | Virus Res | 2021-10 | 共同通讯 |
| 46 | Delivery of microRNA-21-sponge and pre-microRNA-122 by MS2 virus-like particles to therapeutically target hepatocellular carcinoma cells | Exp Biol Med | 2021-12 | 通讯作者 |
| 47 | Comparison of analytical sensitivity of SARS-CoV-2 molecular detection kits | Int J Infect Dis | 2021-08 | 共同通讯 |
| 48 | Detection of Epstein-Barr virus encoded RNA in fixed cells and tissues using CRISPR/Cas-mediated RCasFISH | Anal Biochem | 2021-07 | 共同通讯 |
| 49 | High-fidelity detection of DNA combining the CRISPR/Cas9 system and hairpin probe | Biosens Bioelectron | 2021-07 | 共同通讯 |
| 50 | Development of novel quality control material based on CRISPR/Cas9 editing and xenografts for MLH1 protein deficiency testing | J Clin Lab Anal | 2021-05 | 共同通讯 |
| 51 | Reliable assessment of BRCA1 and BRCA2 germline variants by next-generation sequencing: a multicenter study | Breast Cancer | 2021-05 | 通讯作者 |
| 52 | Prevalence of Asymptomatic SARS-CoV-2 Infection. | Ann Intern Med | 2021-02 | 共同通讯 |
| 53 | The clinical utility of microsatellite instability in colorectal cancer | Crit Rev Oncol Hematol | 2021-01 | 共同通讯 |
| 54 | Technologies for targeted DNA methylation modifications in cancer: Mechanism and application | Biochim Biophys Acta Rev Cancer | 2021-01 | 共同通讯 |
| 55 | Quality evaluation of molecular diagnostic tests for astrovirus, sapovirus and poliovirus: A multicenter study | Clin Chim Acta | 2021-01 | 共同通讯 |
| 56 | External Quality Assessment for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Clinical laboratories | J Mol Diagn | 2021-01 | 共同通讯 |
| 57 | Immunologic testing for SARS-CoV-2 infection from the antigen perspective | J Clin Microbiol | 2021-04 | 共同通讯 |
| 58 | VarBen: Generating in silico Reference Datasets for Clinical Next-Generation Sequencing Bioinformatics Pipeline Evaluation | J Mol Diagn | 2021-03 | 共同通讯 |
| 59 | An Initial Survey of the Performances of Exome Variant Analysis and Clinical Reporting Among Diagnostic Laboratories in China | Front Genet | 2020-11 | 通讯作者 |
| 60 | Comprehensive next-generation profiling of clonal hematopoiesis in cancer patients using paired tumor-blood sequencing for guiding personalized therapies | Clin Transl Med | 2020-11 | 共同通讯 |
| 61 | The miR-21 potential of serving as a biomarker for liver diseases in clinical practice | Biochem Soc Trans | 2020-10 | 通讯作者 |
| 62 | Choosing tumor mutational burden wisely for immunotherapy: A hard road to explore | Biochim Biophys Acta Rev Cancer | 2020-02 | 共同通讯 |
| 63 | Proliferating cell nuclear antigen (PCNA) overexpression in hepatocellular carcinoma predicts poor prognosis as determined by bioinformatic analysis | Chin Med J (Engl) | 2020-11 | 通讯作者 |
| 64 | COVID-19: Insight into the asymptomatic SARS-COV-2 infection and transmission | Int J Biol Sci | 2020-07 | 共同通讯 |
| 65 | Multicenter assessment of microbial community profiling using 16S rRNA gene sequencing and shotgun metagenomic sequencing | J Adv Res | 2020-07 | 共同通讯 |
| 66 | CRISPR/Cas systems redefine nucleic acid detection: Principles and methods | Biosens Bioelectron | 2020-07 | 共同通讯 |
| 67 | Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2 | Theranostics | 2020-06 | 通讯作者 |
| 68 | Molecular and serological assays for SARS-CoV-2: insights from genome and clinical characteristics | Clin Chem | 2020-05 | 共同通讯 |
| 69 | Circulating tumour DNA: A new biomarker to monitor resistance in NSCLC patients treated with EGFR-TKIs | Biochim Biophys Acta Rev Cancer | 2020-04 | 共同通讯 |
| 70 | Liquid biopsy for infectious diseases: a focus on microbial cell-free DNA sequencing | Theranostics | 2020-03 | 共同通讯 |
| 71 | Continual Improvement of the Reliability of EML4-ALK Rearrangement Detection in Non-Small-Cell Lung Cancer: A Long-Term Comparison of ALK Detection in China | J Mol Diagn | 2020-03 | 通讯作者 |
| 72 | Utilizing CRISPR/Cas9 technology to prepare lymphoblastoid cell lines harboring genetic mutations for generating quality control materials in genetic testing | J Clin Lab Anal | 2020-04 | 共同通讯 |
| 73 | External Quality Assurance of Current Technology for the Testing of Cancer-Associated Circulating Free DNA Variants | Pathol Oncol Res | 2020-03 | 共同通讯 |
| 74 | From Somatic Variants Toward Precision Oncology: An Investigation of Reporting Practice for Next-Generation Sequencing-Based Circulating Tumor DNA Analysis | Oncologist | 2020-02 | 通讯作者 |
| 75 | Assessment of significant procedures in multigene molecular detection for breast cancer in clinical laboratories: from variant detection to targeted therapy | Breast Cancer | 2020-01 | 共同通讯 |
| 76 | RCasFISH: CRISPR /dCas9- mediated In Situ imaging of mRNA transcripts in fixed cells and tissues | Anal Chem | 2020-01 | 共同通讯 |
| 77 | Role of miRNA Sponges in Hepatocellular Carcinoma | Clin Chim Acta | 2020-01 | 通讯作者 |
| 78 | Standardization of BCR-ABL1 quantification on the international scale in China using locally developed secondary reference panels | Exp Hematol | 2020-01 | 通讯作者 |
- 科研获奖Research Awards
| # | 获奖证书编号 | 奖项名称 | 获奖级别 | 获奖类别 | 获奖等级 | 获奖日期 | 颁奖单位 | 本单位是否为第一完成单位 | 完成单位排名 | 本人排序 | 备注 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2021-J07-1-01-R03 | 重大病毒性传染病防控产品研发支撑平台和评价关键技术创新和应用 | 省部级 | 科技进步奖、哲学社科奖 | 一等奖 | 2022-11-23 | 北京市人民政府 | 否 | 3 | 3 | |
| 2 | No.2013医-2-005 | 临床免疫和分子诊断质控品及国家标准物质的研究与应用 | 省部级 | 科技进步奖、哲学社科奖 | 二等奖 | 2013-12-01 | 北京市科委 | 是 | 1 | 1 |
- 研究成果Research Findings
| # | 成果类型 | 项目/专利/新品种名称 |
|---|---|---|
| 1 | 作为主创人员获得授权的发明专利和新品种 | 一种肺癌检测质控品及其制备方法 |
| 2 | 作为主创人员获得授权的发明专利和新品种 | 一种检测沙眼衣原体的质控物 |
| 3 | 作为主创人员获得授权的发明专利和新品种 | 快速检测HIV病毒的核苷酸序列、方法及体外诊断试剂盒 |
| 4 | 作为主创人员获得授权的发明专利和新品种 | 一种乳腺癌检测质控品及其制备方法 |
| 5 | 作为主创人员获得授权的发明专利和新品种 | 一种病毒RNA检测引物的设计方法及试剂盒 |
| 6 | 作为主创人员获得授权的发明专利和新品种 | 一种检测人血清杂合轻链抗体的方法及试剂盒 |
| 7 | 作为主创人员获得授权的发明专利和新品种 | 一种检测肿瘤相关基因突变的质控物及其制备方法 |
| 8 | 作为主创人员获得授权的发明专利和新品种 | 检测抗甲状腺过氧化物酶和甲状腺球蛋白的天然双特异性抗体的试剂盒 |
| 9 | 作为主创人员获得授权的发明专利和新品种 | 检测抗环瓜氨酸肽和抗兔免疫球蛋白G双特异性抗体的试剂盒 |
| 10 | 作为主创人员获得授权的发明专利和新品种 | 戊型肝炎兔-人嵌合抗体质控物的制备方法 |
| 11 | 出版高水平专著 | 临床酶免疫测定技术 |
| 12 | 出版高水平专著 | 新型冠状病毒感染临床检测技术 |
| 13 | 出版高水平专著 | 高通量测序技术 |
| 14 | 出版高水平专著 | 实时荧光PCR技术 |
| 15 | 出版高水平专著 | 实时荧光PCR技术(第二版） |
| 16 | 作为主创人员获得授权的发明专利和新品种 | 一种肿瘤突变负荷检测质控品及其制备方法 |