导师风采
杨启文

个人信息

Personal Information

  • 性别:男
  • 导师类型:博导
  • 职称:研究员
  • 该职称任职时间:2004-08-16

联系方式

Contact Information

  • 所属院系:北京协和医院
  • 所属专业:临床检验诊断学
  • 邮箱 : yangqiwen81@vip.163.com
  • 工作电话 : 18600100521

个人简介

Personal Profile

杨启文,研究员,博士生导师,北京协和医学院医学博士,国家“万人计划”科技创新领军人才,研究领域为临床重要致病细菌的耐药致病机制和遗传进化规律,以及病原微生物新型检测技术的研发和转化。构建了国际领先、国内一流的遏制细菌耐药研究体系并组建了5个成熟的研究平台,包括全国多中心临床病原菌耐药及分子流行病学监测平台、病原菌耐药机制及致病机制研究平台、新型抗菌药物临床评价平台、新型快速细菌耐药实验室诊断研发平台、抗菌药物耐药实验室检测标准化平台。在国内开创性地组织了多项大规模细菌耐药监测及分子流行病学研究;发现多种细菌耐药/致病新靶标;作为团队核心牵头组建了我国第一个抗菌药物敏感性试验折点研究国际组织ChiCAST,设立自主研发新药药敏折点以及适用于中国人群的药敏折点,填补行业空白,得到国内外同行的高度认可。至2023年6月主持和参与科研课题70余项,发表中英文论文184篇,其中第一作者和通讯作者SCI文章70篇,中文核心43篇,发表于Lancet Microbe,Clinical Chemistry等杂志,单篇引用最高348次。牵头发布卫生行业专家共识4部。以主要完成人获得省部级科技奖6项。著书参编20本。在研发和转化领域,研发成功细菌毒力/耐药快速检测技术、微生物核酸检测微流控芯片技术等产品,授权中国发明专利30项,国际发明专利2项,实用新型专利4项,转化的诊断产品直接经济效益超千万元,并在国际国内多地应用于临床一线。杨启文教授的系列临床和研究工作提高了我国感染性疾病精准诊断能力,有效遏制了抗菌药物滥用和耐药菌对社会公共健康的威胁。

团队简介

Team Profile

杨启文,研究员,博士生导师,北京协和医学院医学博士,国家“万人计划”科技创新领军人才,研究领域为临床重要致病细菌的耐药致病机制和遗传进化规律,以及病原微生物新型检测技术的研发和转化。构建了国际领先、国内一流的遏制细菌耐药研究体系并组建了5个成熟的研究平台,包括全国多中心临床病原菌耐药及分子流行病学监测平台、病原菌耐药机制及致病机制研究平台、新型抗菌药物临床评价平台、新型快速细菌耐药实验室诊断研发平台、抗菌药物耐药实验室检测标准化平台。在国内开创性地组织了多项大规模细菌耐药监测及分子流行病学研究;发现多种细菌耐药/致病新靶标;作为团队核心牵头组建了我国第一个抗菌药物敏感性试验折点研究国际组织ChiCAST,设立自主研发新药药敏折点以及适用于中国人群的药敏折点,填补行业空白,得到国内外同行的高度认可。至2023年6月主持和参与科研课题70余项,发表中英文论文184篇,其中第一作者和通讯作者SCI文章70篇,中文核心43篇,发表于Lancet Microbe,Clinical Chemistry等杂志,单篇引用最高348次。牵头发布卫生行业专家共识4部。以主要完成人获得省部级科技奖6项。著书参编20本。在研发和转化领域,研发成功细菌毒力/耐药快速检测技术、微生物核酸检测微流控芯片技术等产品,授权中国发明专利30项,国际发明专利2项,实用新型专利4项,转化的诊断产品直接经济效益超千万元,并在国际国内多地应用于临床一线。杨启文教授的系列临床和研究工作提高了我国感染性疾病精准诊断能力,有效遏制了抗菌药物滥用和耐药菌对社会公共健康的威胁。

  • 研究方向Research Directions
病原微生物;致病/耐药机制;遗传进化规律
  • 在校研究生Current Graduate Students

硕士研究生 3 名,博士研究生 1 名

  • 科研项目Research Projects
# 项目名称 起止日期 金额 项目类型 本人角色
1plt-1基因增强ST11型碳青霉烯耐药肺炎克雷伯菌毒力的机制研究2021-01-01 —— 2024-12-3155.0主持在研的国家或省部级科研项目主持者
2基于微流控和单细胞拉曼技术的快速病原菌诊断体系建立及应用研究2019-08-01 —— 2023-07-0194.0参与在研的国家或省部级科研项目参与者
3复杂样品病原体筛查与示踪关键技术研究2021-12-01 —— 2024-11-30340.0主持在研的国家或省部级科研项目主持者
4精准检验平台建设2022-10-01 —— 2025-09-01主持的企业/行业委托重大项目主持人
5突发急性和烈性传染病临床救治关键技术研究/重要院内感染耐药菌防控技术研究2018-08-01 —— 2021-06-01主持在研的国家或省部级科研项目主持人
6重要院内感染耐药菌临床防控技术研究2018-08-01 —— 2021-06-01主持在研的国家或省部级科研项目主持人
7复杂样品病原体筛查与示踪关键技术研究2021-12-01 —— 2024-11-01主持在研的国家或省部级科研项目主持人
8基于单细胞多组学及宿主互作网络的肺炎克雷伯菌黏菌素异质性耐药机制研究2023-01-01 —— 2026-12-31主持在研的国家或省部级科研项目主持人
9plt-1基因增强ST11型碳青霉烯耐药肺炎克雷伯菌毒力的机制研究2021-01-01 —— 2024-12-31主持在研的国家或省部级科研项目主持人
  • 发表论文Papers
# 论文题目 期刊名称 发表年份 论文署名
1侵袭性真菌病的非培养诊断方法。中华检验医学杂志2022-06-09杨启文
22008年北京协和医院细菌耐药性监测结果。中国感染与化疗杂志2022-06-09杨启文
319家医院大肠埃希菌和肺炎克雷伯菌中TEM型β-内酰胺酶的研究。临床检验杂志2022-06-09杨启文
4头孢噻利对铜绿假单胞菌和甲氧西林耐药金葡菌的体外抑菌效应研究中国感染与化疗杂志2022-06-09杨启文
5Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China.Emerg Microbes Infect2022-06-07杨启文
6抗菌药物敏感性试验最新动向:2010年CLSI M100-S20主要更新内容. 中华检验医学杂志2022-06-09杨启文
7Emergence of ST11-K47 and ST11-K64 hypervirulent carbapenem- resistant Klebsiella pneumoniae in bacterial liver abscesses from China: a molecular, biological, and epidemiological study. Emerg Microbes Infect. 2022-06-07杨启文
8Evaluation of the Clinical Systems for Polymyxin Susceptibility Testing of Clinical Gram-Negative Bacteria in China. Front Microbiol. 2022-06-07杨启文
9利奈唑胺对肠球菌属和葡萄球菌属的体外抗菌活性研究。中华检验医学杂志2022-06-09杨启文
10Carbapenem susceptibilities of Gram-negative pathogens in intra-abdominal and urinary tract infections: updated report of SMART 2015 in China. BMC Infect Dis. 2022-06-07杨启文
11Diagnosis of Coxiella burnetii infection via metagenomic next-generation sequencing: a case report.BMC Infect Dis2022-06-07杨启文
12High Prevalence of Extended-Spectrum Beta-Lactamases in Escherichia coli Strains Collected From Strictly Defined Community-Acquired Urinary Tract Infections in Adults in China: A Multicenter Prospective Clinical Microbiological and Molecular Study. Front.Microbiol.2022-06-07杨启文
13肺炎链球菌鼻咽部定植及胞内感染的致病机制研究进展。中国新药杂志2022-06-09杨启文
14全自动智能染色机对临床微生物标本染色性能的评估。中国防痨杂志2022-06-09杨启文
15社区呼吸道感染肺炎链球菌对头孢妥仑的耐药机制研究。中国感染与化疗杂志2022-06-09杨启文
162005年中国十五家教学医院院内获得革兰阴性病原菌耐药性分析中华医学杂志2022-06-09杨启文
172014年北京协和医院细菌耐药性监测。中国感染与化疗杂志2022-06-09杨启文
18荧光免疫层析法降钙素原检测试剂盒临床性能评估。协和医学杂志2022-06-09杨启文
19卫生部全国细菌耐药监测网2011年ICU来源细菌耐药监测中国临床药理学杂志2022-06-09杨启文
202015年全国Vitek2细菌药敏检测系统药敏试验结果准确性调查研究。中华医院感染学杂志2022-06-09杨启文
21Comparative evaluation of four phenotypic methods for detection of class A and B carbapenemase-producing Enterobacteriaceae in China. J Clin Microbiol. 2022-06-07杨启文
22 Antimicrobial susceptibility of Gram-negative bacteria causing intra-abdominal infections in China: SMART China 2011.Chin Med J (Engl).2022-06-09杨启文
23 High prevalence of plasmid-mediated quinolone resistance genes qnr and aac(6’)-Ib-cr in clinical isolates of Enterobacteriaceae from nine teaching hospitals in China.Antimicrob Agents Chemother2022-06-09杨启文
24Analysis of Susceptibilities of Carbapenem Resistant Enterobacterales to Colistin in Intra-Abdominal, Respiratory and Urinary Tract Infections from 2015 to 2017.Infect Drug Resist.2022-06-07杨启文
25In Vitro Activities of Ceftaroline/Avibactam, Ceftazidime/Avibactam, and Other Comparators Against Pathogens From Various Complicated Infections in China. Clin Infect Dis.2022-06-09杨启文
26Comparison of five commonly used automated susceptibility testing methods for accuracy in the China Antimicrobial Resistance Surveillance System (CARSS) hospitals. Infect Drug Resist. 2022-06-09杨启文
27Antimicrobial susceptibility of bacterial pathogens associated with community-acquired respiratory tract infections in Asia: report from the Community-Acquired Respiratory Tract Infection Pathogen Surveillance (CARTIPS) study, 2009-2010. Int J Antimicrob Agents2022-06-09杨启文
28Molecular Epidemiology of Clinical Isolates of Carbapenem-Resistant Acinetobacter spp. from Chinese Hospitals.Antimicrob Agents Chemother2022-06-09杨启文
29Brain Abscess Caused by Bacillus megaterium in an Adult Patient. Chin Med J (Engl).2022-06-09杨启文
30In vitro activity of meropenem combined with vaborbactam against KPC-producing Enterobacteriaceae in China. J Antimicrob Chemother.2022-06-07杨启文
31欧洲临床微生物和感染病大会2018精要概述。协和医学杂志2022-06-09杨启文
32北京协和医院2000~2013年血培养病原菌分布及耐药性分析.检验医学与临床2022-06-09杨启文
33头孢妥仑对社区获得性呼吸道感染流感嗜血菌及黏膜炎莫拉菌的体外抗菌活性中华医院感染学杂志2022-06-09杨启文
34革兰阴性杆菌16SrRNA甲基化酶耐药研究进展检验医学与临床2022-06-09杨启文
35改良Hodge试验在肠杆菌科菌碳青霉烯酶检测中的性能评估.中华检验医学杂志2022-06-09杨启文
36药敏试验折点的设定及对临床的指导意义.内科急危重症杂志2022-06-09杨启文
37头孢米诺等抗菌药物对大肠埃希菌、肺炎克雷伯菌及拟杆菌属的体外抗菌活性.. 中华检验医学杂志2022-06-09杨启文
38环丙沙星或阿米卡星联合β內酰胺类抗生素对多重耐药铜绿假单胞菌的体外联合抑菌效应研究. 中国实用内科杂志2022-06-09杨启文
39血培养阳性菌的分布与报警时间分析。中华医院感染学杂志2022-06-09杨启文
40E试验法检测氨苄西林-舒巴坦,克林霉素,头孢哌酮的体外抗菌活性。中国感染与化疗杂志2022-06-09杨启文
411557株酵母菌的鉴定及其药敏试验分析。中国真菌学杂志2022-06-09杨启文
42头孢噻利对临床常见细菌体外抗菌活性的多中心研究中国感染与化疗杂志2022-06-09杨启文
432004—2005年中国社区获得呼吸道感染常见病原菌耐药性研究中国感染与化疗杂志2022-06-09杨启文
44利奈唑胺、替加环素、达托霉素、头孢吡普等抗菌药物对MRSA的抗菌活性.中国感染与化疗杂志2022-06-09杨启文
452008-2011年北京协和医院流感嗜血杆菌耐药性监测. 中国感染与化疗杂志2022-06-09杨启文
46In vitro activity of cefditoren and other comparators against Streptococcus pneumonia, Haemophilus influenza, and Moraxella catarrhalis causing community-acquired respiratory tract infections in China.Diagn Microbiol Infect Dis. 2022-06-07杨启文
47In vitro Activity of a New Fourth-Generation Cephalosporin, Cefoselis, Against Clinically Important Bacterial Pathogens in China. Front Microbiol.2022-06-07杨启文
48 Antimicrobial susceptibility of Pseudomonas aeruginosa in China: a review of two multicentre surveillance programmes, and application of revised CLSI susceptibility breakpoints. Int J Antimicrob Agents2022-06-08杨启文
49Genetic and Phenotypic Characterization of the Novel Metallo-β-Lactamase NDM-29 From Escherichia coli. Front Microbiol. 2022-06-07杨启文
50 Antimicrobial resistance comparison of Klebsiella pneumoniae pathogens isolated from intra-abdominal and urinary tract infections in different organs, hospital departments and regions of China between 2014 and 2017.J Microbiol Immunol Infect.2022-06-07杨启文
51Antimicrobial susceptibility changes of Escherichia coli and Klebsiella pneumoniae intra-abdominal infection isolate-derived pathogens from Chinese intra-abdominal infections from 2011 to 2015. Infect Drug Resist2022-06-07杨启文
52Clinical and Laboratory Characteristics of Patients with Nontuberculous Mycobacterium Bloodstream Infection in a Tertiary Referral Hospital in Beijing, China.Chin Med J (Engl). 2022-06-09杨启文
53Preliminary Study on Clinical Features and CT Findings of Common-type Coronavirus Disease 2019 Patients in Peking Union Medical College Hospital]Zhongguo Yi Xue Ke Xue Yuan Xue Bao.2022-06-09杨启文
54Outbreak of colonization by carbapenemase-producing Klebsiella?pneumoniae in a neonatal intensive care unit: Investigation, control?measures and assessment.Am J Infect Control.2022-06-09杨启文
55中国7所教学医院耐甲氧西林金黄色葡萄球菌的分子流行病学及耐药性研究。中国感染与化疗杂志2022-06-09杨启文
56Performance Evaluation of BD Phoenix NMIC-413 Antimicrobial Susceptibility Testing Panel for Imipenem, Meropenem, and Ertapenem Against Clinical Carbapenem-Resistant and Carbapenem-Susceptible Enterobacterales. ront Med (Lausanne).2022-06-07杨启文
57全国10所医院院内与社区感染常见病原菌耐药性分析。中华医院感染杂志2022-06-09杨启文
58 中国教学医院细菌耐药监测研究及现状介绍.中国临床药理学杂志2022-06-09杨启文
59两性霉素B和伏立康唑对临床真菌的体外抗菌活性分析。中国真菌学杂志2022-06-09杨启文
605种国产与进口E-test药敏条的一致性比较。临床检验杂志2022-06-09杨启文
61OXA-51样D类碳青霉烯酶在鲍曼不动杆菌中广泛分布中华检验医学杂志2022-06-09杨启文
62 3种常见非发酵革兰阴性杆菌的体外药物敏感性. 中国感染与化疗杂志2022-06-09杨启文
63Clinical perspective of antimicrobial resistance in bacteria.Infect Drug Resist.2022-06-07杨启文
64Establishment of epidemiological cut-off values for cefoselis, a new fourth-generation cephalosporin, against Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Proteus mirabilis and Pseudomonas aeruginosa. J Antimicrob Chemother. 2022-06-07杨启文
65High in?vitro activity of fidaxomicin against Clostridium difficile isolates from a university teaching hospital in China. J Microbiol Immunol Infect.2022-06-07杨启文
66Evidence-Based Medicine Chapter of China International Exchange and Promotive Association for Medical and Health Care (CPAM); Chinese Research Hospital Association (CRHA). Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19: An eMil Med Res.2022-06-08杨启文
67Characteristics of respiratory virus infection during the outbreak of 2019 novel coronavirus in Beijing. Int J Infect Dis.2022-06-08杨启文
68腹腔感染细菌流行病学调查. 中华普通外科学文献2022-06-09杨启文
69Optimized Sequencing Adaptors Enable Rapid and Real-Time Metagenomic Identification of Pathogens during Runtime of Sequencing.Clin Chem. 2022-06-07杨启文
70 Development of a Fast Raman-Assisted Antibiotic Susceptibility Test (FRAST) for the Antibiotic Resistance Analysis of Clinical Urine and Blood Samples. Anal Chem.2022-06-08杨启文
71新疆地区大肠埃希菌和克雷伯菌中产β-内酰胺酶基因型的研究。中华医院感染学杂志2022-06-09杨启文
72Global trends of antimicrobial susceptibility to ceftaroline and ceftazidime-avibactam: a surveillance study from the ATLAS program (2012-2016).Antimicrob Resist Infect Control.2022-06-07杨启文
73Antimicrobial Activity of Colistin Against Contemporary (2015 – 2017) P. aeruginosa and A. Baumannii Isolates From a Chinese Surveillance Program. Front. Microbiol. 2022-06-07杨启文
74Carbapenem-Resistant Isolates of Klebsiella pneumoniae in China and Detection of a Conjugative Plasmid (blaKPC-2 + qnrB4) and a blaIMP-4. Antimicrob agents and Chemothe2022-06-09杨启文
75细菌及真菌血流感染宏基因组学检测标本预处理方法探索。国际检验医学杂志2022-06-09杨启文
76B族链球菌核酸检测试剂的性能评价。中国医学装备2022-06-09杨启文
77临床微生物实验室真菌检测能力建设基本要求专家共识。中华检验医学杂志2022-06-09杨启文
78达托霉素等抗菌药物对499株血流感染革兰阳性球菌的体外抗菌活性.中华内科杂志2022-06-09杨启文
79 MRSA及临床应对浅议.临床药物治疗杂志2022-06-09杨启文
80 CTX-M酶在北京协和医院临床分离大肠埃希菌中的流行。中国感染与化疗杂志2022-06-09杨启文
81肠杆菌目细菌碳青霉烯酶的实验室检测和临床报告规范专家共识。中国感染与化疗杂志2022-06-09杨启文
82中国碳青霉烯类耐药肠杆菌科细菌的流行病学和防控策略中国执业药师2022-06-09杨启文
83精准病原学检查在感染性疾病诊治中的价值。中国实用外科杂志2022-06-09杨启文
84成人酿脓链球菌导致中毒休克综合征临床分析。中华医院感染学杂志2022-06-09杨启文
85低危人群梅毒螺旋体抗体初筛试验结果分析及流程改进建议。临床检验杂志2022-06-09杨启文
862005-2007年中国社区获得性呼吸道感染常见病原菌耐药性调查。中华医学杂志2022-06-09杨启文
87北京协和医院1990—2009年女性生殖道细菌感染常见病原菌及其耐药性监测.中国抗生素杂志2022-06-09杨启文
88儿童医院超广谱β-内酰胺酶大肠埃希菌的基因型与耐药性分析。中华医院感染学杂志2022-06-09杨启文
89Antimicrobial susceptibilities of aerobic and facultative gram-negative bacilli isolated from Chinese patients with urinary tract infections between 2010 and 2014. BMC Infect Dis. 2022-06-07杨启文
90Effect of formaldehyde exposure on bacterial communities in simulating indoor environments. Sci Rep. 2022-06-08杨启文
91 In vitro activity of sulbactam/durlobactam against clinical isolates of Acinetobacter baumannii collected in China. J Antimicrob Chemother2022-06-07杨启文
92Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19).Clin Infect Dis.2022-06-08杨启文
93Significance of Serology Testing to Assist Timely Diagnosis of SARS-CoV-2 infections: Implication from a Family Cluster.Emerg Microbes Infect.2022-06-08杨启文
94临床微生物实验室自动化现状与进展。中华临床实验室管理电子杂志2022-06-09杨启文
95 In vitro activity of flomoxef and comparators against Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis producing extended-spectrum β-lactamases in China.Int J Antimicrob Agents.2022-06-07杨启文
96 腹股沟淋巴结中分离出一株衣氏放线菌.中华检验医学杂志2022-06-09杨启文
97产超广谱β-内酰胺酶肠杆菌科菌对头孢美唑等抗菌药物的体外敏感性分析。中华医院感染学杂志2022-06-09杨启文
98 In Vitro Activity of Imipenem/Relebactam Against Enterobacteriaceae Isolates Obtained from Intraabdominal, Respiratory Tract, and Urinary Tract Infections in China: Study for Monitoring Antimicrobial Resistance Trends (SMART), 2015-2018Clin Infect Dis2022-06-07杨启文
99 诱导性AmpC酶和结构型AmpC酶的检测与产酶菌株感染的治疗进展.中华检验医学杂志2022-06-09杨启文
100High Prevalence of Extended-Spectrum Beta-Lactamases in Escherichia coli Strains Collected From Strictly Defined Community-Acquired Urinary Tract Infections in Adults in China: A Multicenter Prospective Clinical Microbiological and Molecular Study. Front.Microbiol.2022-06-07杨启文
101Rapid diagnosis of Talaromyces marneffei infection assisted by metagenomic next-generation sequencing in a HIV-negative patient. IDCases. 2022-06-07杨启文
102医学检验所基本标准(试行)符合性研究中国医学装备2022-06-09杨启文
103Carbapenemase detection by NG-Test CARBA 5—a rapid immunochromatographic assay in carbapenem-resistant Enterobacterales diagnosis. Ann Transl Med 2022-06-07杨启文
1042009年中国13家教学医院院内感染病原菌的抗生素耐药性监测. 中华检验医学杂志2022-06-09杨启文
105 China Medical Education Association; Expert Committee on Infectious Diseases, China Medical Education Association. Expert Consensus on Polymyxin Antimicrobial Susceptibility Testing and Clinical Interpretation.Chin Med Sci J.2022-06-07杨启文
106 A ten-year surveillance for antimicrobial susceptibility of Escherichia coli and Klebsiella pneumoniae in community- and hospital-associated intra-abdominal infections in China.J Med Microbiol.2022-06-07杨启文
107Agents of Last Resort: An Update on Polymyxin Resistance.Infect Dis Clin North Am.2022-06-07杨启文
108Antimicrobial activity among gram-positive and gram-negative organisms collected from the Asia-Pacific region as part of the Tigecycline Evaluation and Surveillance Trial: Comparison of 2015 results with previous years.Diagn Microbiol Infect Dis.?2022-06-07杨启文
109Combining comparative genomic analysis with machine learning reveals some promising diagnostic markers to identify five common pathogenic non-tuberculous mycobacteria. Microb Biotechnol2022-06-07杨启文
110Susceptibilities of Gram-negative bacilli from hospital- and community-acquired intra-abdominal and urinary tract infections: a 2016–2017 update of the Chinese SMART study. INFECT DRUG RESIST. 2022-06-07杨启文
111Susceptibility to Imipenem/Relebactam of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates from Chinese Intra-Abdominal, Respiratory and Urinary Tract Infections: SMART 2015 to 2018. Infect Drug Resist2022-06-07杨启文
112Update of incidence and antimicrobial susceptibility trends of Escherichia coli and Klebsiella pneumoniae isolates from Chinese intra-abdominal infection patients. BMC Infect Dis.2022-06-08杨启文
113 In vitro and in vivo Effect of Antimicrobial Agent Combinations Against Carbapenem-Resistant Klebsiella pneumoniae with Different Resistance Mechanisms in China. Infect Drug Resist.2022-06-07杨启文
114Emergent Polymyxin Resistance: End of an Era? Open Forum Infect Dis. 2022-06-07杨启文
115A national survey on fungal infection diagnostic capacity in the clinical mycology laboratories of tertiary care hospitals in China. J Microbiol Immunol Infect. 2022-06-08杨启文
1162005—2010年北京协和医院细菌耐药性监测中国感染与化疗杂志2022-06-09杨启文
117Novel transferable resistance-nodulation-division pump gene cluster tmexCD2-toprJ2 that confers tigecycline resistance in Raoultella ornithinolytica. Antimicrob Agents Chemother.2022-06-08杨启文
118Antimicrobial Susceptibilities of Aerobic and Facultative Gram-negative Bacilli from Intra-abdominal Infections in Patients from Seven Regions in China: The 2012-2013 Study for Monitoring Antimicrobial Resistance Trends (SMART). Antimicrob Agents Chemother.2022-06-08杨启文
119In vitro activity of meropenem combined with vaborbactam against KPC-producing Enterobacteriaceae in China. J Antimicrob Chemother.2022-06-07杨启文
120Collateral sensitivity to pleuromutilins in vancomycin-resistant Enterococcus faecium. Nat Commun. 2022-06-08杨启文
121临床重要耐药菌感染传播防控策略专家共识。中国感染控制杂志2022-06-09杨启文
122瑞莱TZ-301型仪器对降钙素原检测能力的临床评价。检验医学与临床2022-06-09杨启文
123Multicenter assessment of shotgun metagenomics for pathogen detection.EBioMedicine.2022-06-08杨启文
124Flomoxef showed excellent in vitro activity against clinically important gram-positive and gram-negative pathogens causing community- and hospital-associated infections.Diagn Microbiol Infect Dis. 2022-06-07杨启文
125Using Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Complemented with Selected 16S rRNA and gyrB Genes Sequencing to Practically Identify Clinical Important Viridans Group Streptococci (VGS). Front Microbiol.2022-06-07杨启文
126Surveillance for aerobic and facultative Gram-negative bacilli isolated from patients with intra-abdominal infections in China: the 2002-2009 Study for Monitoring Antimicrobial Resistance Trends (SMART)Int J Antimicrob Agents2022-06-07杨启文
127Antimicrobial Susceptibility and Virulence of mcr-1-Positive Enterobacteriaceae in China, a Multicenter Longitudinal Epidemiological Study. Front Microbiol. 2022-06-07杨启文
128Emergence of a Plasmid-Encoded Resistance-Nodulation-Division Efflux Pump Conferring Resistance to Multiple Drugs, Including Tigecycline, in Klebsiella pneumoniae.Mbio2022-06-08杨启文
129中国14家教学医院2005-2008年临床分离肺炎链球菌耐药性分析. 中华检验医学杂志2022-06-09杨启文
130Multicenter Evaluation of Xpert Carba-R Assay for detection and identification of the carbapenemase genes in rectal swabs and clinical isolates. J Mol Diagn2022-06-07杨启文
131北京协和医院2008年至2010年血培养菌群分布协和医学杂志2022-06-09杨启文
132The tcdA negative and tcdB positive Clostridium difficile ST81 clone exhibits high-level fluoroquinolone resistance: a multi-center study in Beijing, China. Int J Antimicrob Agents. 2022-06-08杨启文
133北京协和医院1990-2009年住院患者尿道感染常见病原菌及其耐药性。协和医学杂志2022-06-09杨启文
134Phenotypic and genotypic characterization of Enterobacteriaceae with decreased susceptibility to carbapenems: results from large hospital-based surveillance studies in China.Antimicrob Agents and Chemother2022-06-07杨启文
135Active Surveillance of Carbapenemase-Producing Organisms (CPO) Colonization With Xpert Carba-R Assay Plus Positive Patient Isolation Proves to Be Effective in CPO Containment. Front Cell Infect Microbiol.2022-06-07杨启文
136Determination of norvancomycin epidemiological cut-off values (ECOFFs) for Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus hominis.J Antimicrob Chemother.2022-06-07杨启文
137Comprehensive Pathogen Identification, Antibiotic Resistance, and Virulence Genes Prediction Directly From Simulated Blood Samples and Positive Blood Cultures by Nanopore Metagenomic Sequencing.Front Genet. 2022-06-07杨启文
138Clinical Performance of BD Kiestra InoqulA Automated System in a Chinese Tertiary Hospital.Infect Drug Resist.2022-06-07杨启文
139抗菌药物PK/PD参数及合理用药.中国全科医学.2022-06-09杨启文
140Clinical Performance Evaluation of VersaTrek 528 Blood Culture System in a Chinese Tertiary Hospital.Front Microbiol.2022-06-07杨启文
141中国14家教学医院院内菌血症与肺炎和腹腔感染病原菌的抗生素耐药监测中华检验医学杂志2022-06-09杨启文
142Emergence of Ceftazidime- and Avibactam-Resistant Klebsiella pneumoniae Carbapenemase-Producing Pseudomonas aeruginosa in China.mSystems.2022-06-08杨启文
143 An Improved In-house MALDI-TOF MS Protocol for Direct Cost-Effective Identification of Pathogens from Blood Cultures.Front Microbiol.2022-06-07杨启文
144非免疫抑制患者内源性土曲霉眼内炎1例及实验研究。中国真菌学杂志2022-06-09杨启文
145不动杆菌属多重耐药及泛耐药的分子机制研究中华医学杂志2022-06-09杨启文
146碳青霉烯类耐药的不动杆菌分子流行病学及其泛耐药的分子机制中华检验医学杂志2022-06-09杨启文
147替加环素对414株需氧革兰阴性和革兰阳性临床分离菌株的体外抗菌活性中国感染与化疗杂志2022-06-09杨启文
148Genomic epidemiology study of Klebsiella pneumoniae causing bloodstream infections in China.Clin Transl Med. 2022-06-07杨启文
1492008—2011年北京协和医院重症监护病房细菌耐药性监测协和医学杂志2022-06-09杨启文
150High ceftaroline non-susceptibility in Staphylococcus aureus isolated from acute skin infections in 15 tertiary hospitals in China.J Med Microbiol.2022-06-08杨启文
1512013-2018年北京协和医院4种常见性传播疾病病原体检出情况。协和医学杂志2022-06-09杨启文
152Epidemiology of carbapenem-resistant?Klebsiella pneumoniae bloodstream infections after renal transplantation from donation after cardiac death in a Chinese hospital: a case series analysis. Antimicrob Resist Infect Control.2022-06-09杨启文
153Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae causing intra-abdominal infections from 9 tertiary hospitals in China. Diagn Microbiol Infect Dis.2022-06-09杨启文
154Endocarditis caused by Arcanobacterium pyogenes. Chin Med J (Engl). 2022-06-09杨启文
155Collaborated effort against SARS-CoV-2 outbreak in China. Clin Transl Med. 2022-06-07杨启文
156 Involvement of MarR and YedS in carbapenem resistance in a clinical isolate of Escherichia coli from China. Antimicrob Agents Chemother.2022-06-09杨启文
157多黏菌素药物敏感性检测及临床解读专家共识。协和医学杂志2022-06-09杨启文
158抗菌药物敏感性试验折点的解读与应用. 中华检验医学杂志2022-06-09杨启文
159腹腔感染大肠埃希菌和肺炎克雷伯菌的耐药性监测。中国感染与化疗杂志2022-06-09杨启文
1602008年中国15所教学医院医院感染患者分离的肠杆菌科细菌耐药性分析。中国感染与化疗杂志2022-06-09杨启文
161依替米星体外抗菌活性及其与β-内酰胺类抗菌药物的协同作用研究.中华医院感染学杂志2022-06-09杨启文
162嗜麦芽窄食单胞菌体外药物敏感性研究.中国感染与化疗杂志2022-06-09杨启文
163北京协和医院外科住院患者细菌耐药性监测。协和医学杂志2022-06-09杨启文
164格特隐球菌在河北地区引起1例脑膜炎的临床与实验研究。中国真菌学杂志2022-06-09杨启文
1652010年CLSI三代头孢菌素折点改变对我国大肠埃希菌和肺炎克雷伯菌及奇异变形杆菌药物敏感性结果解释的评估。中华检验医学杂志2022-06-09杨启文
166氟康唑体外抗菌活性及五种体外敏感性试验方法的比较中华检验医学杂志2022-06-09杨启文
1672010年北京协和医院细菌耐药性监测中国感染与化疗杂志2022-06-09杨启文
168CHINET2011年北京协和医院细菌耐药性监测中国感染与化疗杂志2022-06-09杨启文
169Susceptibilities of Gram-negative bacilli from hospital- and community-acquired intra-abdominal and urinary tract infections: a 2016–2017 update of the Chinese SMART studyINFECT DRUG RESIST2019-04-01通讯作者
170Active Surveillance of Carbapenemase-Producing Organisms (CPO) Colonization With Xpert Carba-R Assay Plus Positive Patient Isolation Proves to Be Effective in CPO Containment.Frontiers in Cellular and Infection Microbiology2019-05-14通讯作者
171Clinical Performance of BD Kiestra InoqulA Automated System in a Chinese Tertiary HospitalINFECTION AND DRUG RESISTANCE2020-04-01通讯作者
172Antimicrobial Susceptibility and Virulence of mcr-1-Positive Enterobacteriaceae in China, a Multicenter Longitudinal Epidemiological Study.Frontiers in Microbiology2020-07-28通讯作者
173In vitro activity of sulbactam/durlobactam against clinical isolates of Acinetobacter baumannii collected in ChinaJOURNAL OF ANTIMICROBIAL CHEMOTHERAPY2020-07-01通讯作者
174Antimicrobial susceptibility changes of Escherichia coli and Klebsiella pneumoniae intra-abdominal infection isolate-derived pathogens from Chinese intra-abdominal infections from 2011 to 2015INFECTION AND DRUG RESISTANCE2019-08-01通讯作者
175Genetic and Phenotypic Characterization of the Novel Metallo-β-Lactamase NDM-29 From Escherichia coliFrontiers in Microbiology2021-08-31通讯作者
176In vitro Activity of a New Fourth-Generation Cephalosporin, Cefoselis, Against Clinically Important Bacterial Pathogens in ChinaFRONTIERS IN MICROBIOLOGY2020-02-01通讯作者
177Antimicrobial Activity of Colistin Against Contemporary (2015-2017)P.aeruginosa and A.baumannii Isolates From a Chinese Surveillance ProgramFRONTIERS IN MICROBIOLOGY2020-09-01通讯作者
178Analysis of Susceptibilities of Carbapenem Resistant Enterobacterales to Colistin in Intra-Abdominal, Respiratory and Urinary Tract Infections from 2015 to 2017INFECTION AND DRUG RESISTANCE2020-07-01通讯作者
179High Prevalence of Extended-Spectrum Beta-Lactamases in Escherichia coli Strains Collected From Strictly Defined Community-Acquired Urinary Tract Infections in Adults in China: A Multicenter Prospective Clinical Microbiological and Molecular StudyFrontiers in Microbiology2021-07-07通讯作者
180Establishment of epidemiological cut-off values for cefoselis, a new fourth-generation cephalosporin, against Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Proteus mirabilis and Pseudomonas aeruginosaJOURNAL OF ANTIMICROBIAL CHEMOTHERAPY2021-06-03通讯作者
181In vitro and in vivo Effect of Antimicrobial Agent Combinations Against Carbapenem-Resistant Klebsiella pneumoniae with Different Resistance Mechanisms in ChinaInfection and Drug Resistance2021-03-05通讯作者
182Performance Evaluation of BD Phoenix NMIC-413 Antimicrobial Susceptibility Testing Panel for Imipenem, Meropenem, and Ertapenem Against Clinical Carbapenem-Resistant and Carbapenem-Susceptible Enterobacteralesfrontiers in medicine2021-04-14通讯作者
183Carbapenemase detection by NG-Test CARBA 5—a rapid immunochromatographic assay in carbapenem-resistant Enterobacterales diagnosisAnnals of Translational Medicine2021-05-05通讯作者
184Combining comparative genomic analysis with machine learning reveals some promising diagnostic markers to identify five common pathogenic non-tuberculous mycobacteriaMicrobial Biotechnology2021-05-21通讯作者
185Comprehensive Pathogen Identification, Antibiotic Resistance, and Virulence Genes Prediction Directly From Simulated Blood Samples and Positive Blood Cultures by Nanopore Metagenomic SequencingFrontiers in Microbiology2021-03-24通讯作者
186Evaluation of the Clinical Systems for Polymyxin Susceptibility Testing of Clinical Gram-Negative Bacteria in ChinaFrontiers in Microbiology2021-02-22通讯作者
187Multicenter Evaluation of Xpert Carba-R Assay for Detection and Identification of the Carbapenemase Genes in Rectal Swabs and Clinical IsolatesJOURNAL OF MOLECULAR DIAGNOSTICS2021-01-23通讯作者
188Determination of norvancomycin epidemiological cut-off values (ECOFFs) for Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus hominisJOURNAL OF ANTIMICROBIAL CHEMOTHERAPY2021-01-01通讯作者
189Antimicrobial resistance comparison of Klebsiella pneumoniae pathogens isolated from intra-abdominal and urinary tract infections in different organs, hospital departments and regions of China between 2014 and 2017JOURNAL OF MICROBIOLOGY,IMMUNOLOGY AND INFECTION2020-03-19通讯作者
190Susceptibility to Imipenem/Relebactam of Pseudomonas aeruginosa and Acinetobacter baumannii Isolates from Chinese Intra-Abdominal, Respiratory and Urinary Tract Infections: SMART 2015 to 2018.Infection and Drug Resistance2021-08-31通讯作者
191Optimized Sequencing Adaptors Enable Rapid and Real-Time Metagenomic Identification of Pathogens during Runtime of SequencingCLINICAL CHEMISTRY2022-06-01通讯作者
192Agents of Last Resort: An Update on Polymyxin ResistanceINFECTIOUS DISEASE CLINICS OF NORTH AMERICA2020-12-01通讯作者
193Emergence of colistin-resistant hypervirulent Klebsiella pneumoniae (CoR-HvKp) in China.Emerging Microbes & Infections2022-12-01通讯作者
194Global trends of antimicrobial susceptibility to ceftaroline and ceftazidime-avibactam: a surveillance study from the ATLAS program (2012-2016)Antimicrobial Resistance and Infection Control2020-10-27通讯作者
195Emergence of ST11-K47 and ST11-K64 hypervirulent carbapenem- resistant Klebsiella pneumoniae in bacterial liver abscesses from China: a molecular, biological, and epidemiological study.Emerging Microbes & Infections2020-09-09通讯作者
196Coexistence of blaNDM-1 and blaIMP-4 in One Novel Hybrid Plasmid Confers Transferable Carbapenem Resistance in an ST20-K28 Klebsiella pneumoniae. Frontiers in Microbiology2022-03-31通讯作者
197Xu Y. Antimicrobial Activity of Colistin Against Contemporary (2015 – 2017) P. aeruginosa and A. Baumannii Isolates From a Chinese Surveillance Program. Frontiers in Microbiology2020-09-04通讯作者
198Emergence of a Superplasmid Coharboring Hypervirulence and Multidrug Resistance Genes in Klebsiella pneumoniae Poses New Challenges to Public HealthMicrobiology Spectrum2022-12-21通讯作者
199Background Filtering of Clinical Metagenomic Sequencing with a Library Concentration-Normalized ModelMicrobiology Spectrum2022-09-22通讯作者
200Development and clinical evaluation of an online automated quality control system for improving laboratory quality managementCLINICA CHIMICA ACTA2023-02-15通讯作者
201Artificial intelligence-aided rapid and accurate identification of clinical fungal infections by single-cell Raman spectroscopyFrontiers in Microbiology2023-03-22通讯作者
202A-to-I RNA Editing in Klebsiella pneumoniae Regulates Quorum Sensing and Affects Cell Growth and Virulence.Advanced Science2023-04-21通讯作者
203Antimicrobial susceptibility to polymyxin B and other comparators against Gram-negative bacteria isolated from bloodstream infections in China: Results from CARVIS-NET programFrontiers in Microbiology2022-10-06通讯作者
204Emergence of a Novel NDM-5-Producing Sequence Type 4523 Klebsiella pneumoniae Strain Causing Bloodstream Infection in China.Microbiology Spectrum2022-10-26通讯作者
205In vitro activity of ceftaroline, ceftazidime-avibactam, and comparators against Gram-positive and -negative organisms in China: the 2018 results from the ATLAS programBMC MICROBIOLOGY2022-10-01通讯作者
206Emergence of ST463 exoU-Positive, Imipenem-Nonsusceptible Pseudomonas aeruginosa Isolates in ChinaMicrobiology Spectrum2023-06-14通讯作者
207In-vitro activity of ceftolozane/tazobactam against Pseudomonas aeruginosa collected in the Study for Monitoring Antimicrobial Resistance Trends (SMART) between 2016-2019 in ChinaJOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY2023-02-01通讯作者
208Clinical Perspective of Antimicrobial Resistance in BacteriaINFECTION AND DRUG RESISTANCE2022-03-02通讯作者
209Emergent Polymyxin Resistance: End of an Era?OPEN FORUM INFECTIOUS DISEASES2019-10-01通讯作者
210Application of metagenomic sequencing of drainage fluid in rapid and accurate diagnosis of postoperative intra-abdominal infection: a diagnostic studyInternational Journal of Surgery2023-09-01通讯作者
211Antimicrobial Susceptibility Among Gram-Negative Isolates in Pediatric Patients in Latin America, Africa-Middle East, and Asia From 2016-2020 Compared to 2011-2015: Results From the ATLAS Surveillance StudyJournal of the Pediatric Infectious Diseases Society2023-08-10通讯作者
212Effect of ceftazidime-avibactam combined with different antimicrobials against carbapenem-resistant Klebsiella pneumoniaeMicrobiology Spectrum2024-05-07通讯作者
213Novel transferable resistance-nodulation-division pump gene cluster tmexCD2-toprJ2 that confers tigecycline resistance in Raoultella ornithinolyticaANTIMICROBIAL AGENTS AND CHEMOTHERAPY2021-03-18第一作者
214In Vitro Activity of Imipenem/Relebactam Against Enterobacteriaceae Isolates Obtained from Intraabdominal, Respiratory Tract, and Urinary Tract Infections in China: Study for Monitoring Antimicrobial Resistance Trends (SMART), 2015-2018CLINICAL INFECTIOUS DISEASES2020-12-23第一作者
215Emergence of a Plasmid-Encoded Resistance-NodulationDivision Efflux Pump Conferring Resistance to Multiple Drugs, Including Tigecycline, in Klebsiella pneumoniaemBio2020-03-03第一作者
216Multicenter assessment of shotgun metagenomics for pathogen detection.EBioMedicine2021-12-01第一作者
217 China Medical Education Association; Expert Committee on Infectious Diseases, China Medical Education Association. Expert Consensus on Polymyxin Antimicrobial Susceptibility Testing and Clinical Interpretation.Chinese Medicine2021-03-01第一作者
218Collaborated effort against SARS-CoV-2 outbreak in ChinaCLINICAL AND TRANSLATIONAL MEDICINE2020-03-01第一作者
  • 科研获奖Research Awards
# 获奖证书编号 奖项名称 获奖级别 获奖类别 获奖等级 获奖日期 颁奖单位 本单位是否为第一完成单位 完成单位排名 本人排序 备注
120120601中华医学会第七次全国中青年检验医学学术会议优秀论文国家级其他国家级奖励三等奖2012-06-01中华医学会11
2201106012011年中华医学会第三届感染与抗微生物治疗论坛暨第九届全国感染性疾病及抗微生物化疗学术会议青年优秀论文国家级其他国家级奖励三等奖2011-06-01中华医学会第三届感染与抗微生物治疗论坛11
320190601中国医师协会检验医师年会暨第十四届全国检验与临床学术会议优秀论文国家级其他国家级奖励一等奖2019-06-01中国医师协会检验医师年会11
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  • 研究成果Research Findings
# 成果类型 项目/专利/新品种名称
1作为主创人员获得授权的发明专利和新品种机器视觉鉴定分析及药敏鉴定系统
2作为主创人员获得授权的发明专利和新品种一种菌种留取保存运输管
3作为主创人员获得授权的发明专利和新品种一种带自驱动单元的微流控芯片、微流控方法及其应用
4作为主创人员获得授权的发明专利和新品种一种可多步时序反应的微流控芯片、微流控方法及其应用
5作为主创人员获得授权的发明专利和新品种医学检测用高清图像采集装置
6作为主创人员获得授权的发明专利和新品种一种重组DNA聚合酶及其制备方法和应用
7作为主创人员获得授权的发明专利和新品种视觉鉴定分析及药敏鉴定系统
8作为主创人员获得授权的发明专利和新品种一种突变重组逆转录酶及其制备方法和应用
9作为主创人员获得授权的发明专利和新品种用于检测大肠埃希菌和肺炎克雷伯菌及其毒性和耐药性的核酸试剂、试剂盒、系统及方法
10出版高水平专著临床检验结果案例解析
11出版高水平专著中国抗菌药物管理和细菌耐药现状报告(2019)
12出版高水平专著临床真菌学图谱(第一部)
13出版高水平专著中国抗菌药物管理和耐药现状报告(2020)
14出版高水平专著临床真菌学图谱(第二部)
15出版高水平专著细菌真菌感染多学科诊疗思维释例
16出版高水平专著抗微生物药物敏感性试验体系EUCAST应用工具篇
17出版高水平专著抗微生物药物敏感性试验体系-EUCAST标准操作规程篇
18出版高水平专著细菌药物敏感试验执行标准和典型报告解读
19出版高水平专著病原宏基因组测序的临床应用
20作为主创人员获得授权的发明专利和新品种一种可多步时序反应的微流控芯片、微流控方法及其应用
21作为主创人员获得授权的发明专利和新品种一种重组DNA聚合酶及其制备方法和应用
22作为主创人员获得授权的发明专利和新品种一种预测肺炎克雷伯菌对头孢吡肟敏感性的方法及系统
23作为主创人员获得授权的发明专利和新品种病原微生物宏基因组生信分析参考品及其制备方法和应用
24作为主创人员获得授权的发明专利和新品种一种带自驱动单元的微流控芯片、微流控方法及其应用(香港)
25作为主创人员获得授权的发明专利和新品种一种预测肺炎克雷伯菌对头孢曲松敏感性的方法及系统
26作为主创人员获得授权的发明专利和新品种一种预测克雷伯氏菌属对阿米卡星敏感性的系统及方法
27作为主创人员获得授权的发明专利和新品种一种预测克雷伯氏菌属对美罗培南敏感性的系统及方法
28作为主创人员获得授权的发明专利和新品种一种预测克雷伯氏菌属对哌拉西林-他唑巴坦敏感性的系统及方法
29作为主创人员获得授权的发明专利和新品种一种预测克雷伯氏菌属对头孢他啶敏感性的系统及方法
30作为主创人员获得授权的发明专利和新品种一种预测克雷伯氏菌属对头孢西丁敏感性的系统及方法
31作为主创人员获得授权的发明专利和新品种一种预测克雷伯氏菌属对亚胺培南敏感性的系统及方法
32作为主创人员获得授权的发明专利和新品种一种Smad4基因检测生物传感器及制备方法
33作为主创人员获得授权的发明专利和新品种一种微生物保存液及其使用方法
34作为主创人员获得授权的发明专利和新品种一种预测克雷伯氏菌属对左氧氟沙星敏感性的系统及方法
35作为主创人员获得授权的发明专利和新品种一种可多步时序反应的微流控芯片、微流控方法及其应用(香港)
36作为主创人员获得授权的发明专利和新品种基于CE平台的呼吸道病毒和/或细菌亚型引物设计方法及系统
37作为主创人员获得授权的发明专利和新品种一种用于消除培养皿盖内壁上的雾气的方法、装置和设备
38作为主创人员获得授权的发明专利和新品种一种高通量高精度检出多种病原菌的引物组、试剂盒及方法
39作为主创人员获得授权的发明专利和新品种真菌阳性血培养分离胶及化学试剂前处理方法(PRETREATMENT METHOD FOR FUNGAL POSITIVE BLOOD CULTURE BY USING SEPARATING GEL AND CHEMICAL REAGENT)
40作为主创人员获得授权的发明专利和新品种一种构建病原微生物数据鉴定平台的方法及装置
41作为主创人员获得授权的发明专利和新品种一种检测呼吸道病毒病原体的NGS引物组及试剂盒
42作为主创人员获得授权的发明专利和新品种一种带自驱动单元的微流控芯片、微流控方法及其应用
43作为主创人员获得授权的发明专利和新品种用于检测大肠埃希菌和肺炎克雷伯菌及其毒性和耐药性的核酸试剂、试剂盒、系统及方法
44作为主创人员获得授权的发明专利和新品种一种突变重组逆转录酶及其制备方法和应用